Frictionless Ordering System
Personal Information
Please enter your contact information to finish your quote. After clicking "Next", your quote will be emailed to you.
Note that this step does not formalize your order. You will need to enter your sample details to formally create an order.
The data will be shared with the email address provided here.
Our ordering process consists of the following steps:
- Generate a quote. Select your desired services and quantities for a quote that is valid for up to 90 days.
- Enter your contact information. A PDF quote will be emailed to you within a few minutes.
-
Enter your sample details.
Sample details cannot be changed.
- If you need to generate a PO, stop here. After you have your PO, use the link sent alongside the PDF quote to return to your order.
- If you have your PO# or are paying by credit card, provide your sample names (either through the web form or downloadable Excel file) and add additional order details.
- Enter payment information. We accept Purchase Orders or Credit Cards (subject to a 3.3% fee). Orders are not complete and will not be processed without payment information. You are not charged for your order until you receive your data.
- Receive shipping information. You’ll receive instructions on how and where to ship your samples. We deliver your data electronically in 2 weeks or less after receiving all parts of your order (samples, reference files, payment method).
Select your service, add-ons, and review your order.
Service #1
Category
Select a Class to see Categories.
Main Service
Select a Category to see Main Services.
Add-ons
Select a Main Service to see Add-ons.
Orders of 48 samples or more receive 5% off. Excludes PacBio services, dedicated Illumina flowcells, and Ultra-Long and Direct RNA Nanopore services.
Order Summary
| Description | Price | Qty | Total |
|---|
Quote Details
| # | Description | Quantity | Unit Price | Total |
|---|---|---|---|---|
| Final Total | $0 | |||
Sample Details
Pause here
We recommend pausing here and reviewing the next steps. You cannot change the information entered below after it is submitted. When you are ready to continue, use the link that was just sent by email with your PDF quote to checkout.
Collect the following order information and review the requirements
- Sample names: Sample names can contain only numbers, letters, and underscores “_” (no spaces). Names must match easily or correlate to the physical sample labels.
- Material requirements: Ensure that your samples meet the requirements for concentration, volume, and media by reviewing the information on the service’s page. (Use the “Services” menu to navigate to the category and then individual service page.) If you are unsure, please contact info@seqcenter.com. Samples sent below requirements will be processed as they are and will be billed in full.
- Payment method: Secure your purchase order (PO) from your purchasing department or have your credit card ready. You must enter this information to complete the checkout process and access the shipping information page.
Continue placing the order
Once you have done the above, please use one of the two options below to enter your sample details. For samples with extraction add-ons, please put 0 for the concentration.
Option 1: By template
Download and complete the template at your convenience. You can return and upload your completed template to auto-populate the table below.
Option 2: By Webform
Enter your sample information and measurement method below. Please navigate the table using the 'Tab' key.
Additional Sample Information
Please complete the table below to indicate which samples are receiving additional services and upload your references if necessary.
Please provide details for each sample.
Reference Files
Please upload your reference files for your analyses. We accept the following file formats: .gbk, .gb, .gff, .gbff, .fasta, .fa, .fna, and compressed versions of these formats. If your reference is a published genome, you can enter the NCBI Accession number in the comments box below.
-
Variant calling analysis can accept all file types, but will not contain gene information if a fasta format
is provided (
.fasta, .fa, .fna). -
RNA Basic and Intermediate analysis requires either a
.gbk,.gb,.gff, or.gbfffile or NCBI assembly accession number. - Metatranscriptome services can bioinformatically remove host reads with any of the accepted file types. If these samples are from human, mouse, or the environment, please state this in the Comments box instead.
Overall Comments
For all orders: If your order includes a negative control sample and you wish to receive data for it, please specify which samples are the controls and confirm your request to receive the data, even if the amount is less than the requested package size. The full package price will be charged for these samples. Samples named as negative controls or blanks will also have this policy applied.
For RNA orders: If you have previously ordered custom rRNA depletion probes and would like to use them for this order, you must note them in the comments box below.
For Intermediate RNA Analysis, please list which pairwise comparisons you would like to have.
For HiFi PacBio offerings: Please include the genus/species NCBI taxonomic ID for each sample for annotation. (If you would like a partially or fully-phased assembly instead of the complimentary collapsed assembly, please contact us at info@seqcenter.com).
Payment Method
Billing Information
If you are ready to complete your purchase, enter your payment information below.
For POs, please enter your institution's invoicing email or your own if you will be submitting the invoice yourself.
Please note that credit card payments are subject to a 3.3% fee.
After submitting your billing information, you will be automatically taken to the shipping information for your order.
If you are not ready to pay, you can return by clicking "Access a Quote" from our main page.
Please do not ship your samples until you have finalized the order with payment information.
Overall Notes
Sample Details
When submitting your samples please ensure they meet the following requirements:
- All purified nucleic acid samples should be resuspended in nuclease-free water or dilute Tris or TE buffer. (Most extraction kit buffers are OK.)
- Samples should be sent in microcentrifuge tubes labeled with permanent marker or sealed 96-well PCR plates for larger submissions. (Please send PCR plates on dry ice to reduce the possibility of leaking or cross contamination.)
- We ask customers to not use sticker labels, as they are prone to fall off.
- When shipping with dry ice, include at least 5–10 lbs of dry ice per day of expected transit. Do NOT ship Thursday or Friday.
- Concentration values below correspond to Qubit or other dye-based methods. If using a Nanodrop, at least double is required.
Extractions
Generally, 109 cells are required either as aspirated cell pellets, well-grown lawns on agar, or homogenized tissue. For exact requirements, see the extraction section of the specific service page.
DNA, Illumina Material Requirements
| WGS, WES, and Shotgun Metagenome | 16S (V3-V4) / ITS2 | Full Flowcells | Hybrid Sequencing | |
|---|---|---|---|---|
| Volume | ≥ 30 μL | ≥ 20 μL | ≥ 30 μL, single pool |
Two aliquots are required: 1 meeting Illumina WGS reqs. 1 meeting ONT Ligation reqs. |
| Qubit quantification | ≥ 10 ng/μL | No minimum | ≥ 1 ng/μL, final pool | |
| Shipping conditions | Ambient | Ambient | Dry ice | |
| Other | Fragment length ≥ 400 bp | If using custom primers, please send 10–20 μL at 100 μM of each. |
DNA, Long Read Material Requirements
| ONT Ligation | Nanopore Ultra-Long | PacBio WGS | Full-Length 16S | |
|---|---|---|---|---|
| Volume | ≥ 60 μL | 750 μL | ≤ 100 μL | ≥ 20 μL |
| Qubit quantification | ≥ 40 ng/μL | ≥ 40 ng/μL | ≥ 2 μg total | ≥ 1 ng/μL |
| Shipping conditions | Cool packs or dry ice, overnight recommended | |||
| Other | Must have no contact with Axygen consumables |
RNA Material Requirements
| Illumina RNA and Metatranscriptomics | Nanopore Direct RNA (poly-A only) | PacBio Iso-Seq (poly-A only) | |
|---|---|---|---|
| Volume | ≥ 20 μL | ≥ 20 μL | ≤ 7 μL |
| Qubit quantification | ≥ 50 ng/μL |
≥ 15 ng/μL (pre-depleted) ≥ 50 ng/μL (undepleted) |
≥ 500 ng mRNA total |
| Shipping conditions | Dry ice, overnight required | ||
| RIN Recommendation | RIN > 6 | RIN > 8 | RIN > 7 |
| Other | Must have no contact with Axygen consumables |
Specialized Service Material Requirements
| PacBio AAV | Illumina SARS-CoV-2 | |
|---|---|---|
| Volume | ≤ 50 μL | ≥ 15 μL RNA or ≥ 250 μL whole sample in VTM |
| Qubit quantification | ≥ 500 ng total | None, but CT value < 35 |
| Shipping conditions | Dry ice, overnight required | |
| Other | Must have no contact with Axygen consumables |
If your samples are unable to meet these requirements due to the nature of your organism, please contact us to see if custom library preparation is available.
* Please see our FAQ for more information about the differences in Qubit and Nanodrop measurements.
Please send samples to:
SeqCenter
91 43rd Street, Suite 250
Pittsburgh, PA 15201, USA
For additional shipping questions, please contact us at info@seqcenter.com.
Select Add-ons
Click "Save" to apply your selection.
Quote Not Found
The quote you're looking for doesn't exist or has expired. Please start a new order to continue.