Frictionless Ordering System

Personal Information

If you are requesting a quote only: enter your contact information and select your services. The quote will be sent to you by email. You can stop at the Summary page.

If you are placing an order: you must complete the entire checkout process in one session. Please prepare all required information in advance, including your payment information. Details of what is required by service can be found here: https://www.seqcenter.com/required-information/

Frictionless Ordering System

Quote Information

Please enter the contact information that should appear on your quote.

Instructions for selecting services:

Start by characterizing the samples and their origins with the type and class dropdown menus. The categorization does not need to be exact, but is meant to help guide your selection.
- Single organism, includes isolates/pure cultures, tissue or blood samples, polymorphic populations
- Multi-organism: Host + pathogen, Environmental samples, Mixed community
- Construct: for prepared sequencing libraries that have sequencing adapters, prepared PCR amplicons for long read sequencing
Choose a service category and then a specific package size.
- For PacBio services, only the library preparations and their prices are shown. Flowcell entries will be added automatically to your list when adding a prep.
Select add-on services for all samples in this group. They will be added to your list of services at the bottom of the page.
- Some add-ons have additional add-ons themselves. Be sure to select all of the Extras using the checkboxes before adding the primary add-on to your quote with the “+Add” button.
Adjust the quantities using the boxes at the bottom of the page. Quantities for add-on services must be the same as the number of samples for a main service.
- If not all samples should receive the same add-on services, please split them into a separate service group.
- PacBio customers, please adjust the number of preps to the number of samples you have and flowcells to meet your sequencing needs. (You can multiplex more than 1 sample per flowcell.) If you are unsure of how many flowcells you need, please contact us with your organism, service, number of samples, and target coverage/depth.
To add another service group of samples, click the “+Add Another Service” button and repeat the process.
- To remove a service group, click the red ‘x’ in the top right corner of the service box.
Once you are happy with the list of services, click Next to generate your quote. Bulk discounts will be calculated at that time.

Tip: Our catalog is large! There may be a delay for options to load. If you encounter an error, please attempt to reselect the category and service.

If you have any questions, please contact us at info@seqcenter.com.

Select your service, add-ons, and review your order.

Service #1

Type

Class

Main Service

Select a Category to see Main Services.

Add-ons

Select a Main Service to see Add-ons.

Orders of 48 samples or more receive 5% off. Excludes PacBio services, dedicated Illumina flowcells, and Ultra-Long and Direct RNA Nanopore services.

Order Summary

Description	Price	Qty	Total

Order Total $0

Order Details

#	Description	Quantity	Unit Price	Discount	Total
Final Total					$0

Sample Details

Before you begin

Please ensure that you have all information that is required to check out, including your payment information. The rest of the process must be completed in one session. You will not be able to exit and return during this process.

To continue

Please enter the details specific to the material that you are sending by either entering the information directly into the form below sample-by-sample or using the template option to fill out the data in a tabular format. The template option is recommended for orders with many samples.

Option 1: By template

Download and complete the template using Excel following the instructions below. Keep this window open.

Once completed, upload the file in .xlsx or .csv format to populate the table below and then review. Fields that remain blank may have a data type mismatch. Please refer to our field explanations for additional help.

Certain fields require exact value matches. Please see this section in our field explanations for values that can be copied and additional details.

Option 2: By Webform

Enter your sample information and measurement method below. Please navigate the table using the 'Tab' key.

Additional Sample Information

Please complete the table below to indicate which samples are receiving additional services and upload your references if necessary.

Please provide details for each sample.

Overall Comments

Please include any additional comments you may have. If you have questions that you would like addressed before we receive the samples, please contact us directly at info@seqcenter.com in advance.

To have your data shared with additional collaborators, please include their email addresses or Globus IDs in a request below.

For all orders with negative controls: If you wish to receive data for the negative controls, please:

specify which samples are the controls
confirm your request to receive the data, even if the amount is less than the requested package size.

The full package price will be charged for these samples, no matter the output. Samples named as negative controls or blanks will also have this policy applied.

For services with reference files: If you provided more than 1 file, please indicate which samples should use which reference files.

Payment Method

Warning! In order to save your sample information, you must complete this last step. (This is a change from our previous system.)

For Purchase Orders:

Please enter the information from your Purchase Order into the fields below, including the email address(es) where the invoice should be sent.

If you are still waiting for your PO, please enter “Purchase Order in progress” as the PO# to complete the only process. You must contact finance@seqcenter.com once you have your PO. SeqCenter will not begin work until a PO is received.

For credit card payments:

Please note that credit card payments are subject to a 3.3% processing fee.

If you have your credit card present, you can use the “Credit Card” option to enter it. An authorization will be opened on your card. Payment is not captured until work is completed.
If a payment link should be sent by email instead, please choose the “Payment Link” option and enter the recipient’s email address. You may be asked to prepay for services.

Payment Method

Order Total: $0

By checking this box, you are agreeing to our Terms of Service and Privacy Policies.

Order #: ORD2015594

Purchase Date: 07/09/25

Payment Method:

Customer Name:

Institution:

Email:

Phone:

Address:

Bill to:

Please save a digital copy of this page and print a physical copy to include in your package. You will not be able to return to this page.

Measurement Method:

Overall Notes

Sample Details

When submitting your samples please ensure they meet the following requirements:

All purified nucleic acid samples should be resuspended in nuclease-free water or dilute Tris or TE buffer. (Most extraction kit buffers are OK.)
Samples should be sent in microcentrifuge tubes labeled with permanent marker or sealed 96-well PCR plates for larger submissions. (Please send PCR plates on dry ice to reduce the possibility of leaking or cross contamination.)
We ask customers to not use sticker labels, as they are prone to fall off.
When shipping with dry ice, include at least 5–10 lbs of dry ice per day of expected transit. Do NOT ship Thursday or Friday.
Concentration values below correspond to Qubit or other dye-based methods. If using a Nanodrop, at least double is required.

Extractions

Generally, 10⁹ cells are required either as aspirated cell pellets, well-grown lawns on agar, or homogenized tissue. For exact requirements, see the extraction section of the specific service page.

DNA, Illumina Material Requirements

	WGS, WES, and Shotgun Metagenome	16S (V3-V4) / ITS2	Full Flowcells	Hybrid Sequencing
Volume	≥ 30 μL	≥ 20 μL	≥ 30 μL, single pool	Two aliquots are required: 1 meeting Illumina WGS reqs. 1 meeting ONT Ligation reqs.
Qubit quantification	≥ 10 ng/μL	No minimum	≥ 1 ng/μL, final pool
Shipping conditions	Ambient	Ambient	Dry ice
Other	Fragment length ≥ 400 bp		If using custom primers, please send 10–20 μL at 100 μM of each.

DNA, Long Read Material Requirements

	ONT Ligation	Nanopore Ultra-Long	PacBio WGS	Full-Length 16S
Volume	≥ 60 μL	750 μL	≤ 100 μL	≥ 20 μL
Qubit quantification	≥ 40 ng/μL	≥ 40 ng/μL	≥ 2 μg total	≥ 1 ng/μL
Shipping conditions	Cool packs or dry ice, overnight recommended
Other			Must have no contact with Axygen consumables

RNA Material Requirements

	Illumina RNA and Metatranscriptomics	Nanopore Direct RNA (poly-A only)	PacBio Iso-Seq (poly-A only)
Volume	≥ 20 μL	≥ 20 μL	≤ 7 μL
Qubit quantification	≥ 50 ng/μL	≥ 15 ng/μL (pre-depleted) ≥ 50 ng/μL (undepleted)	≥ 500 ng mRNA total
Shipping conditions	Dry ice, overnight required
RIN Recommendation	RIN > 6	RIN > 8	RIN > 7
Other			Must have no contact with Axygen consumables

Specialized Service Material Requirements

	PacBio AAV	Illumina SARS-CoV-2
Volume	≤ 50 μL	≥ 15 μL RNA or ≥ 250 μL whole sample in VTM
Qubit quantification	≥ 500 ng total	None, but CT value < 35
Shipping conditions	Dry ice, overnight required
Other	Must have no contact with Axygen consumables

If your samples are unable to meet these requirements due to the nature of your organism, please contact us to see if custom library preparation is available.
* Please see our FAQ for more information about the differences in Qubit and Nanodrop measurements.

Please send samples to:
SeqCenter
91 43rd Street, Suite 250
Pittsburgh, PA 15201, USA

For additional shipping questions, please contact us at info@seqcenter.com.

Frictionless Ordering System

Personal Information

Frictionless Ordering System

Quote Information

Instructions for selecting services:

Service #1

Category

Main Service

Add-ons

Order Summary

Order Details

Sample Details

Before you begin

To continue

Option 1: By template

Option 2: By Webform

Additional Sample Information

Reference Files for Analysis Add-ons

Overall Comments

Payment Method

Overall Notes

Sample Details

When submitting your samples please ensure they meet the following requirements:

Extractions

DNA, Illumina Material Requirements

DNA, Long Read Material Requirements

RNA Material Requirements

Specialized Service Material Requirements

Select Add-ons

Quote Not Found